• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The fungi belonging to the Coriolus genus are extracted by using a dilute alkaline solution having a concentration within a certain specified range whereby the low molecular weight substances with molecular weight of less than 5,000 are obtained in there extract solution. These low molecular weight substances are separated from the extract solution to obtain a nitrogen-containing polysaccharide having excellent anti-tumor activity.
    公开号:SU730277A3
    申请号:SU772472207
    申请日:1977-04-20
    公开日:1980-04-25
    发明作者:Уэно Сабуро；Есикуми Чикао；Хиросе Фумио；Омура Есио；Вада Тосихико；Фудзии Такаеси；Такахаси Эйичи
    申请人:Куреха Кагаку Когио Кабусики Кайся (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to the chemical and pharmaceutical industry and relates to the preparation of medicinal preparations. A method of producing nitrogen-containing polysaccharides with antitumor activity is known by extracting basidiomycetes from fungi of the class with a hot extractant with the following isolation and purification of the target product 1. However, with this method, the yield of the target product is insignificant - 3.5%. target product. This is achieved in that the extraction is carried out from the fungus Coriolus genus of Polyporaceae with an aqueous solution of alkali at a concentration of 0.01-2 N. and purification are ultrafiltration and / or reversible osmosis. In addition, Coriolus Versicolor (Fr) Quel a is taken from the fungi belonging to the Coriolus genus of Polyporaceae as an aqueous alkali solution, sodium hydroxide solution is used in an amount exceeding the fungi 5–200 times (by weight). Ultrafiltration is carried out under a pressure of 0.5-5 kg / cm, and reversible osmosis at a pressure of 25-35 kg / cm. The method is carried out as follows. The fungi are extracted from the basidiomycete class of Coriolus genus of Polyporaceae with an aqueous solution of alkali at a concentration of 0.01-2 N. At a temperature of 50-110 ° C, preferably 80-98 ° C, for 20-600 minutes. Various alkaline compounds such as sodium hydroxide, potassium hydroxide, ammonium hydroxide or calcium hydroxide are used to prepare the alkaline solution, preferably potassium and sodium hydroxide is used. The resulting liquid extract is neutralized by a conventional method using a mineral acid, for example, diluted hydrochloric acid, and then this neutralized extract is subjected to ultrafiltration or reversible osmosis to (Obtain low molecular weight substances (mol% less than 5000) in the extract. For this product component fractionated under pressure by molecular weight using a membrane which is a type of molecular sieve.
    The ultrafiltration process is carried out under a pressure of 0.5-5 kg / cm, preferably 1-4 g / cm 3, and at a temperature of 5-70 ° C.
    In the case of reversible osmosis, a pressure in the order of 2035 kg / cm, preferably 20 to 25 kg / cm, is applied at a temperature of 5-20 ° C.
    Ultrafiltration is used to fractionate a material with a molecular weight in excess of iOOOO, and reversible osmosis is applicable to the fractionation of a material with a molecular weight less than 1000.
    The extract, from which substances with a low molecular weight (less than 5000) are removed, is spray dried or lyophilized and then converted into marketable products.
    The polysaccharides obtained are reddish brown in color and contain nitrogen in the amount of 2–8%, in a number of cases 3–6%. They do not have a clear melting point and gradually darken and decompose at temperatures above 1200 s.
    Polissarchids are soluble in water, almost insoluble in alcohol, pyridine, chloroform, benzene and hexane, have no taste or smell.
    The molecular weight of the polysaccharides is between 10,000 and 100,000.
    Nitrogen-containing polysaccharides have high anti-bcfyxolevoy activity with a high retention rate against α-sarcoma-80 in mice with intraperitoneal administration, but also ensure the effectiveness of treatment and oral administration.
    The resulting nitrogen-containing polysaccharides can be used to prevent side effects in cancer chemotherapy. Or increase the effectiveness of radiotherapy and prevent infectious diseases caused by viruses or bacteria, improve liver function, appetite, regulate internal disorders and maintain urological functions.
    Example 1. To 200 g of a dry mycelium of Coriolus Versicolor (Fr) Quel (FERM - P No. 2414) add 4 liters Ofl and. NaOH solution and extracted with stirring in a boiling water bath at a temperature of the extracted mass of EO-55 C for 1 hour; and then cooled to 50 ° C and 1N solution is added in portions, HSE to maintain a pH of 7.0. The solid phase is then removed by filtration under vacuum and the resulting solid is washed with 500 ml of water to give 4.2 liters of liquid extract. This liquid extract is subjected to ultrafiltration using a special ultrafilter with stirring and cooling, wire filtration under pressure of 1/5 kt / cm at 10 ° C with separation of low-molecular weight compounds with molecular weight less than 5000 and subsequent concentration, obtaining 300 m 26.6 g of a reddish-brown powder (13.5% yield) was isolated. This powder contains 7.5% moisture and has the following elemental analysis: C — 40.5%, H — 6.2%, N — 5.8% O — 47.5% (the percentage of oxygen is determined by subtracting 100 total percentage of the remaining elements). This substance is easily soluble in water and has about 90% - a retention factor against a solid tumor of sarcoma-180 in mice with intraperitoneal administration and 65% - when administered orally.
    The anti-tumor effect of the compounds is determined by the usual method. Sarcoma-180 tumor cells are transplanted into the abdominal cavity of a mouse, and after these cells have grown sufficiently within 7 days, 10 such cells are transplanted under the skin of the other mouse, where a solid tumor forms.
    Treatment with a drug that is tested is started 24 hours after transplantation. In the case of intraperitoneal treatment, the drug is administered at a dose of 10 mg / kg per course of treatment once a day, or every second day for 20 days with a total dose of 0.2 ml / 20 live weight of the mouse, and in the case of oral administration, the drug is administered at a dose 1000 mg / kg per treatment, i.e. once a day for 20 days with a total dose of 0.2 ml / 20 g of live weight of the mouse. A tumor is formed in the tumor on the 25th day after transplantation, the tumor growth inhibition rate is calculated from the average tumor weight of the mouse treated with the preparation and the average tumor weight of the control mouse. For comparison, extraction and purification are carried out under the same conditions, but using water instead of 4 liters of 0.1 N. NaOH solution. The product yield is 7.8% or about 60% of the yield obtained according to the method of the invention.
    Example 2. To 500 g of Coriolus versicolor (Fr) Quel FERM - P No. 2414 live mycelium (moisture content 70.8%, total nitrogen, 2.6% based on dry material) add 2 liters of water and grind into a mixer for juice 10-20 min. and 500 ml of 1N NaOH solution was then gradually added to this mixture and extracted for 2 hours in a hot water bath at the temperature of the extracted mass of EO-EB C, then neutralized with hydrochloric acid, washed and treated as described in Example 1. subjected to ultrafiltration on a special ultrafilter to separate low molecular weight p
    权利要求:
    Claims (4)
    [1]
    1. A method of producing nitrogen-containing polysaccharides having an antitumor effect by extraction from Baeidiomycetes class 35 mushrooms with a hot extractant, followed by isolation and purification of the target product, characterized in that, in order to increase the yield of the target product, 40 are extracted from Coriolus genus of mushrooms Poly ^ poraceae with an aqueous solution of alkali at a concentration of 0.01 - 2N, and purification is carried out by ultrafiltration and / or reverse osmosis.
    45
    [2]
    2. The method of pop. 1, characterized in that from the fungi belonging to Coriolus genus of Polyporaceae take Coriolus versicolor (Fr) Quel.
    [3]
    3. The method of pop. 1, which is explained by the fact that a solution of sodium hydroxide is used in an amount of 5-200 times greater than mushrooms (by weight) as an aqueous alkali solution.
    [4]
    “4. The method of π. 1, with the fact that ultrafiltration is carried out under a pressure of 0.5-5 kg / cm, and reversible osmosis is 25-35 kg / cm .
    类似技术:
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    同族专利:
    公开号 | 公开日
    NL169616B|1982-03-01|
    JPS5614274B2|1981-04-03|
    CA1072034A|1980-02-19|
    ES457722A1|1978-03-01|
    AU2416777A|1978-10-19|
    PL197374A1|1978-01-16|
    US4699787A|1987-10-13|
    RO70328A|1981-11-24|
    DK151877A|1978-01-08|
    BG33007A3|1982-11-15|
    SE7703195L|1978-01-08|
    DD130249A5|1978-03-15|
    DE2713458C2|1984-03-15|
    GB1571075A|1980-07-09|
    AR213963A1|1979-04-11|
    DK145505B|1982-11-29|
    US4202969A|1980-05-13|
    CS219880B2|1983-03-25|
    PL102730B1|1979-04-30|
    MX4243E|1982-02-25|
    BR7703439A|1978-03-28|
    FR2361910B1|1983-01-21|
    IN145169B|1978-09-02|
    NL7703434A|1978-01-10|
    YU39795B|1985-04-30|
    CH628649A5|1982-03-15|
    ATA219277A|1980-05-15|
    SE436170B|1984-11-19|
    FR2361910A1|1978-03-17|
    NL169616C|1982-08-02|
    IT1075700B|1985-04-22|
    DE2713458A1|1978-01-19|
    DK145505C|1983-04-25|
    ZA773442B|1978-04-26|
    JPS536412A|1978-01-20|
    AT360145B|1980-12-29|
    YU73777A|1982-08-31|
    DE2759978C2|1987-02-19|
    AU497455B2|1978-12-14|
    BE852987A|1977-09-29|
    HU175947B|1980-11-28|
    PH12242A|1978-12-12|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3436311A|1966-02-28|1969-04-01|Pillsbury Co|Fungal polysaccharide composition and method for making same|
    DE1915687C2|1968-03-28|1982-03-18|Kaken Kagaku K.K., Tokyo|Pharmaceutical preparation with anti-tumor activity|
    US3759896A|1968-03-28|1973-09-18|T Yamamoto|Process for manufacture of polysaccharides with antitumor action|
    US3436346A|1968-06-10|1969-04-01|Pillsbury Co|Process for preparing filterable aqueous polysaccharide solutions|
    BE757248A|1969-10-15|1971-04-08|Kureha Chemical Ind Co Ltd|SUBSTANCE WITH ANTI-CANCER PROPERTY AND METHODS FOR ITS PREPARATION|
    US3754925A|1970-03-24|1973-08-28|Takeda Chemical Industries Ltd|New thermo gelable polysaccharide containing foodstuffs|
    US4051314A|1970-10-14|1977-09-27|Kureha Kagaku Kogyo Kabushiki Kaisha|Polysaccharides and method for producing same|
    US3933788A|1971-11-11|1976-01-20|Kelco Company|Polysaccharide and bacterial fermentation process for its preparation|
    JPS4924211A|1972-07-01|1974-03-04|
    JPS544960B2|1972-07-17|1979-03-12|
    JPS57793B2|1972-09-16|1982-01-07|
    FR2261755B1|1974-02-25|1977-11-04|Thomas Andre|
    JPS5628152B2|1975-12-18|1981-06-30|
    PH14773A|1976-01-01|1981-12-09|Kureha Chemical Ind Co Ltd|Protein-bound polysaccharides|
    JPS5614275B2|1976-07-07|1981-04-03|
    JPS5614276B2|1976-07-22|1981-04-03|
    JPS5726645B2|1977-09-16|1982-06-05|JPS5614275B2|1976-07-07|1981-04-03|
    US4851395A|1976-07-07|1989-07-25|Kureha Kagaku Kogyo Kabushiki Kausha|Nitrogen-containing polysaccharide|
    JPS5614276B2|1976-07-22|1981-04-03|
    JPS5646481B2|1977-01-27|1981-11-04|
    FR2444464B1|1978-12-19|1982-12-31|Fabre Sa Pierre|
    JPS5646793B2|1979-08-08|1981-11-05|
    JPS5932480B2|1981-02-10|1984-08-09|Kureha Chemical Ind Co Ltd|
    AU549856B2|1981-02-27|1986-02-20|Otsuka Pharmaceutical Factory, Inc.|Glycoprotein inhibiting toxoplasma multiplication|
    US4614733A|1981-12-31|1986-09-30|Kureha Kagaku Kogyo Kabushiki Kaisha|Polysaccharides pharmaceutical compositions and the use thereof|
    US4417415A|1982-04-26|1983-11-29|Battelle Development Corporation|Process for culturing a microalga, and extracting a polysaccharide therefrom|
    JPS6328413B2|1982-07-15|1988-06-08|Mutsunori Fujiwara|
    JPS5926109U|1982-08-10|1984-02-18|
    DE3448155C2|1983-08-11|1989-11-02|Kureha Kagaku Kogyo K.K., Nihonbashi, Tokio/Tokyo, Jp|
    CA1339308C|1987-06-18|1997-08-19|Koichi Niimura|Polysaccharides and antiviral drug containing polysaccharides as active ingredient|
    AU599241B2|1987-06-18|1990-07-12|Kureha Kagaku Kogyo Kabushiki Kaisha|Polysaccharides and antiviral drugs containing the same as active ingredient|
    JPH0825890B2|1987-06-18|1996-03-13|呉羽化学工業株式会社|Antiviral agent|
    JPH0643336B2|1988-06-30|1994-06-08|呉羽化学工業株式会社|Vascular growth inhibitor|
    JP2782443B2|1988-12-26|1998-07-30|將純 吉原|Anti-AIDS virus agent|
    CA2156767A1|1994-08-25|1996-02-26|Kenichi Matsunaga|Binding agent for growth factor|
    US7048932B2|2002-05-22|2006-05-23|The Chinese University Of Hong Kong|Preparation and standardization of immunomodulatory peptide-linked glucans with verifiable oral absorbability from coriolus versicolor|
    US7829098B2|2005-11-08|2010-11-09|Vita Green Health Products Co., Ltd|Herbal powder extracts and methods of preparing and using the same|
    US7854936B2|2008-01-14|2010-12-21|Active Organics, Inc.|Anti-inflammatory hydrolysate of C. versicolor |
    CN107823228B|2010-10-06|2021-07-20|寶佳生物科技有限公司|Coriolus versicolor extract and preparation method and application thereof|
    CN103113442B|2013-02-28|2015-04-22|华南理工大学|Method for extracting cordyceps polysaccharide and adenosine from cordyceps sinensis mycelium|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    JP8066476A|JPS5614274B2|1976-07-07|1976-07-07|
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